Member publications

2012

Plant-produced recombinant influenza vaccine based on virus-like HBc particles carrying the extracellular domain of M2 protein. Ravin N.V., Kotlyarov R.Y., Mardanova E.S., Kuprianov V.V., Migunov A.I., Stepanova L.A., Tsybalova L.M., Kiselev O.I., Skryabin K.G. Biochemistry (Mosc); in press. (2012).

Newviral vector for efficient production of epitopes of vaccine proteins in plants. L.G.Tyulkina, E.V. Skurat, O.V. Frolova, T.V. Komarova, E.M. Karger and J.G. Atabekov. Acta Naturae, 3, ╧ 5 (12) in press

2011

Transmissible gastroenteritis coronavirus RNA dependent RNA polymerase and the nonstructural proteins 2, 3 and 8 are incorporated intro viral particles. Nogales, A., Marquez-Jurado, S., Galan, C., Enjuanes, L., and Almazan, F. J. Virol. Submitted (2011).

In planta production of a candidate vaccine against bovine papillomavirus type 1. A. J. Love, S. N. Chapman,  S. Matic, E. Noris, G. P. Lomonossoff  and  M. Taliansky. Plant Cell Reports. Submitted (2011).

Expression of Active Recombinant Human Gastric Lipase in 1 N. Benthamiana using the CPMV2 HT Transient Expression System. Maria Vardakou, Frank Sainsbury, Neil Rigby, Francis Mulholland, George P. Lomonossoff Protein Expression and Purification in press (2011)

The complete nucleotide sequence of Alternanthera mosaic virus infecting Portulaca grandiflora represents a new strain distinct from phlox isolates. Ivanov P.A., Mukhamedzhanova A.A., Smirnov A.A., Rodionova N.P., Karpova O.V., Atabekov J.G. Virus Genes, Vol.42.:268-271 (2011) DOI 10.1007/s11262-010-0556-6

Improved foreign gene expression in plants using a virus-encoded suppressor of RNA silencing modified to be developmentally harmless. Pooja Saxena, Yi-Cheng Hsieh, Veria Y. Alvarado, Frank Sainsbury, Keith Saunders, George P. Lomonossoff, and Herman B. Scholthof. Plant Biotechnology Journal, 9, pp. 703–712 (2011).

Recent advances of cowpea mosaic virus-based particle technology. Nicholas P. Montague, Eva C. Thuenemann, Pooja Saxena, Keith Saunders, Paolo Lenzi and George P. Lomonossoff Human Vaccines 7:3, 1-8 (2011).

A human papillomavirus 8 E7 protein produced in plants is able to trigger the mouse immune system and delay the development of skin lesions. Noris E, Poli A, Cojoca R, Rittà M, Cavallo F, Vaglio S, Matic S, Landolfo S. Arch Virol. 156(4):587-95. (2011).

Immunogenic characterization and epitope mapping of transmissible gastroenteritis virus RNA dependent RNA polymerase. Nogales, A., Galan, C., Marquez-Jurado, S., Garcia-Gallo, M., Kremer, L., Enjuanes, L., and Almazan, F. 2011. J. Virol. Methods 175:7-13 (2011).

Foot and mouth disease virus polyepitope protein produced in bacteria and plants induces protective immunity in guinea pigs.Andrianova E..P, Krementsugskaia S.R., Lugovskaia N.N., Mayorova T.K., Borisov V.V., Eldarov M.A., Ravin N.V., Folimonov A.S., Skryabin KG. Biochemistry (Mosc); 76(3):339-46 (2011).

2010

Vectored vaccines to protect against PRRSV Cruz, J.L.G., Zuñiga, S., Becares, M., Sola, I., Ceriani, J.E., Juanola, S., Plana, J., and Enjuanes, L. Virus Res. 154:150-160 (2010).

Plant production of vaccines [PLAPROVA] George P. Lomonossoff in A decade of EU-funded GMO research (2001-2010) Directorate-General for Research and Innnovation, European Commission http://bookshop.europa.eu/en/a-decade-of-eu-funded-gmo-research-2001-2010--pbKINA24473/ (2010).

2009

Cowpea mosaic virus chimeric particles bearing ectodomain of matrix protein 2 (M2E) of influenza A virus: production and characteristics. Meshcheriakova IuA, El'darov MA, Migunov AI, Stepanova LA, Repko IA, Kiselev OI, Lomonosov GP, Skriabin KG. Mol. Biol. (Mosk). 43(4):741-50. (2009).

Efficient generation of cowpea mosaic virus empty virus-like particles by the proteolytic processing of precursors in insect cells and plants. Keith Saunders, Frank Sainsbury and George P. Lomonossoff. Virology, 393, 329-337. (2009).

Conference Posters and Presentations

2011

Liudmila Tsybalova (INFLRAMS) presented results from the project at the Fourth ESWI influenza conference (Malta, 11-14 September 2011) in the form of poster report named “Candidate M2e-based vaccines against human and avian influenza viruses produced in plants: immune response and protection”. The abstract will be published in one of the issues of Vaccine.

Saxena P., Sainsbury F., Saunders K., Lomonossoff G. P. (2011) Using a modified virus-encoded suppressor of silencing to enhance protein expression in transgenic plants. Plant based vaccines and antibodies conference, Porto, June 8-10, 2011.

EC Thuenemann, AE Meyers, EP Rybicki and GP Lomonossoff. (2011) Transient expression of immunogenic bluetongue VLPs in N. benthamiana. Plant based vaccines and antibodies conference, Porto, June 8-10, 2011.

AE Meyers and EP Rybicki. (2011) BTV protein expression in N. benthamiana using a replicating Geminivirus expression vector. Plant based vaccines and antibodies conference, Porto, June 8-10, 2011.

Becares, M., Cruz, J.L.G., Sanchez, C.M., Ros, S., Juanola, S., Plana, J., Zuñiga, S., and Enjuanes, L. 2011. Design of TGEV derived vectors and antigenic structures to protect against porcine reproductive and respiratory syndrome. 6th International Symposium on Emerging and Re-emerging Pig Diseases. Barcelona, Spain.

Kotlyarov R.Y., Blokhina E.A., Kuprianov V.V., Migunov A.I., Stepanova L.A., Tsybalova L.M., Kiselev O.I., Ravin N.V., Skryabin K.G. (2011) Development of plant-produced recombinant influenza vaccines based on the M2 protein. The 2011 Viruses and Cells Gordon Research Conference, Lucca, Italy.

Blokhina E.A., Kuprianov V.V. (2011) Virus-like nanoparticles based on the core protein of hepatitis B virus and M2 protein of influenza virus as a new candidate influenza vaccine. International conference RUSNANOTECH-2011, Moscow, Russia.

2010

Saxena P., Saunders K., Sainsbury F., Lomonossoff G. P. (2010) Exploiting P19, a virus-encoded suppressor of silencing, to enhance protein expression in transgenic plants. International Advances in Plant Virology Conference, Arnhem, the Netherlands. September 5-7, 2010.

Becares, M., Cruz, J. L. G., Zúñiga, S., Sánchez, C., Ros, S., Juanola, S., Plana, J., and Enjuanes, L. 2010. Improvement of TGEV derived vector to protect against porcine reproductive and respiratory syndrome. International PRRS Symposium. Chicago, IL, USA.

Enjuanes, L. 2010. Coronavirus replication and reverse genetics. Fundamental virology Course, Pasteur Institute. Paris, France.

Enjuanes, L., Sola, I., Almazán, F., Zuñiga., S., Mateos-Gomez, P. A., Nieto-Torres, J. L., Jimenez-Guardeño, J. M., and DeDiego, M. L. 2010. Coronavirus expression vectors, transcription mechanisms and protection against SARS-CoV. 5th International Meeting on Biotechnology, BIOSPAIN 2010. Pamplona, Spain.

Ravin N.V., Kotlyarov R.Y., Kuprianov V.V., Mardanova E.S., Dykov M., Grudinin M.P., Kiselev O.I., Skryabin K.G. (2010) Development of plant viral expression systems for production of influenza virus vaccine proteins in plants. Abstracts of The X European congress of virology, Cernobbio, Italy.

2009

Cruz, J. L. G., Zúñiga, S., Sánchez, C., Ros, S., Juanola, S., Plana, J., and Enjuanes, L. 2009. Design of a TGEV vector to protect against porcine reproductive and respiratory syndrome. International PRRS Symposium. Chicago, IL, USA.

Enjuanes, L. 2009. Coronavirus replication and reverse genetics. Fundamental virology Course, Pasteur Institute. Paris, France.

Enjuanes, L., Sola, I., Zuñiga, S., Mateos-Gómez, P. A., Nieto-Torres, J. L., Jimenez-Guardeño, J. M., and DeDiego, M. L. 2009. Mechanism of transcription in coronavirus and vector development. International Symposium “Fronteras cruciales entre la inmunología y la terapia génica”. Fundación Ramón Areces. Pamplona, Spain.

Ravin N.V., Skryabin K.G., Atabekov J.G. (2009) Production of virus-like nanoparticles carrying immunodominant epitopes of human and animal pathogens in plants. The second international conference on nanotechnology, Moscow, Russia.

A conference (Saint-Petersburg, 28-29 October 2009) was held on the premises of Research Institute of Influenza on new generation vaccines, in which participants from Austria and Kazakhstan took part. Four reports on plant immune preparation were presented, including one report from MSU, one report from CB RAS, and two reports from INFLRAMS RU.